Fig. 2.

Detection of mutagenesis in transformed 41B cells. a Detection of EGFP signal in 41B cells. The cells transformed with CCD8-sgRNA expression construct was given as an example. Those cells with EGFP signal were considered as transformed cells and were used for subsequent analysis. Scale bars: 100 μm. b Sanger sequencing results of the target sites in VvCCD7 and VvCCD8 genes in transformed 41B cells. The wild-type sequences generated clear sequencing chromatograms, whereas the mutated sequences generated overlapping peaks at the mutation sites. The PAM sequences adjacent to CCD7-sgRNA and CCD8-sgRNA are shadowed