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. 2020 Jan 29;11:40. doi: 10.1186/s13287-019-1540-2

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Characterization of primary cultured hDPSCs and ephrinB2 expression in hDPSCs during osteogenic differentiation. a Mesenchymal stem cell markers measured by flow cytometry. b Colony-forming units stained with crystal violet. c Osteogenic, adipogenic, and neurogenic potentials of hDPSCs were confirmed by Alizarin Red S staining, Oil Red O staining, and β III -tubulin expression. Scale bar of left and right images, 100 μm; scale bar of middle images, 20 μm. d Expression of p-ephrinB2, ephrinB2, p-EphB4, and EphB4 in hDPSCs during osteogenic differentiation. Protein expression levels were normalized to that of β-actin. Data are shown as mean ± SD. Assays were repeated three times. *p < 0.05 vs. sample on day 0 of osteogenic induction