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. 2020 Jan 29;11:40. doi: 10.1186/s13287-019-1540-2

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — The effect of endogenous ephrinB2 overexpression on osteogenic differentiation of hDPSCs. a ALP staining on day 7 of osteogenic differentiation. Scale bar = 200 μm. Staining intensity was quantified with ImageJ. b Alizarin Red S staining on days 14, 21, and 28 of osteogenesis. Staining intensity was quantified with ImageJ. c–e mRNA expression of RUNX2, ALP, BMP2, COL1, OCN, DSPP, and DMP1 in EfnB2-hDPSCs (ephrinB2 overexpression) and Vector-hDPSCs (ephrinB2 overexpression control) after 7, 14, and 21 days of osteogenic induction. Data are shown as mean ± SD. Assays were repeated three times. *p < 0.05 and **p < 0.01 vs. Vector-hDPSCs