Table 1.
Species | Gene | Primers |
---|---|---|
Homo sapiens | ephrinB2 |
For: 5′-TATGCAGAACTGCGATTTCCAA-3′ Rev: 5′-TGGGTATAGTACCAGTCCTTGTC-3′ |
ALP |
For: 5′-CCTCGTTGACACCTGGAAGAG-3′ Rev: 5′-TTCCGTGCGGTTCCAGA-3′ |
|
RUNX2 |
For: 5′-TCTTAGAACAAATTCTGCCCTTT-3′ Rev: 5′-TGCTTTGGTCTTGAAATCACA-3′ |
|
BMP2 |
For: 5′-TTCCACCATGAAGAATCTTTGGA-3′ Rev: 5′-CCTGAAGCTCTGCTGAGGTGAT-3′ |
|
COL1 |
For: 5′-GAGGGCCAAGACGAAGACATC-3′ Rev:5′- CAGATCACGTCATCGCACAAC-3′ |
|
OCN |
For: 5′-CTACCTGTATCAATGGCTGGG-3′ Rev: 5′-GGATTGAGCTCACACACCT-3′ |
|
DSPP |
For: 5′-TTTGGGCAGTAGCATGGGC-3′ Rev: 5′-CCATCTTGGGTATTCTCTTGCCT-3′ |
|
DMP1 |
For: 5′-CTCCGAGTTGGACGATGAGG-3′ Rev: 5′-TCATGCCTGCACTGTTCATTC-3′ |
|
EphB4 |
For: 5′-CGCACCTACGAAGTGTGTGA-3′ Rev: 5′-GTCCGCATCGCTCTCATAGTA-3′ |
|
β-actin |
For: 5′-ACGTTGCTATCCAGGCTGTG-3′ Rev: 5′-GGCCATCTCTTGCTCGAAGT-3′ |
|
Canis familiaris | RUNX2 |
For: 5′-TACCACACCTACCTGCCACCAC-3′ Rev: 5′-GCGGAAGCATTCTGGAAGGAGAC-3′ |
BMP2 |
For: 5′-TGAACTCCACTAACCACGCCATTG-3′ Rev: 5′-TGTTGGTACACAGCACGCCTTG-3′ |
|
ephrinB2 |
For: 5′-TGCCAGACAAGAGCCATGAAGATC-3′ Rev: 5′-GGCGTCGTGTTGGATCATTATGC-3′ |
|
β-actin |
For: 5′-ATCACTATTGGCAACGAGCGGTTC-3′ Rev: 5′-CAGCACTGTGTTGGCATAGAGGTC-3′ |
Abbreviations: ALP alkaline phosphatase, RUNX2 runt-related transcription factor 2, BMP2 bone morphogenetic protein 2, COL1 collagen type I, OCN osteocalcin, DSPP dentin sialophosphoprotein, DMP1 dentin matrix protein 1, For forward, Rev reverse