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. 2020 Jan 28;16:1744806920903150. doi: 10.1177/1744806920903150

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© The Author(s) 2020

Creative Commons CC BY: This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Extrinsic sensory innervation of NMR colorectum. (a) Comparison of mouse and NMR gastrointestinal tracts from anus (left) to oesophagus (right), with a 30-cm ruler providing scale. Whole-mount immunostaining for CGRP in the mesentery (b; inset, nerve fibres encircling a mesenteric blood vessel. Arrows, example nerve fibre on the blood vessel margin), myenteric plexi (c; inset, extrinsic nerve fibres infiltrating myenteric ganglia) and submucosa (d) of NMR. Equivalent nerve fibre staining was also observed with PGP9.5 in the mesentery (e; inset, nerve fibre surrounding mesenteric blood vessel), myenteric plexi (f), submucosa (g; inset, nerve fibre surrounding the base of a mucosal villi), circular muscle (h) and longitudinal muscle (i; arrows, nerve fibre innervating longitudinal muscle). Scale bar in each panel: 50 µm. CGRP: calcitonin gene-related peptide; PGP9.5: protein gene product 9.5.