Table 3.
Concentrations of cytokines in plasma of GRdim/dim and GR+/+ mice intrapoeritoneal challenged with lipopolysaccharide (LPS) or vehicle (phosphate buffered saline, PBS) measured at the end of the experiment.
| Parameters | Resuscitation (IV crystalloids and norepinephrine) | |||
|---|---|---|---|---|
| GR+/+ + Vehicle (n = 7–8) | GRdim/dim + Vehicle (n = 6–7) | GR+/+ + LPS (n = 7–8) | GRdim/dim + LPS (n = 10–11) | |
| Il-1 alpha [pg·ml−1] |
27 (12; 65) | 19 (10; 22) | 67 (55; 70) | 67 (22; 68)# |
| Il-2 [pg·ml−1] |
3.5 (1.1; 6.4) | 2.9 (1.6; 6.6) | 16.2 (14.0; 19.2)* | 13.7 (7,5; 17.3) |
| Il-3 [pg·ml−1] |
1.4 (0.8; 2.0) | 1.1 (0.2; 1.6) | 13.1 (12.4; 14.0)* | 12.7 (5.4; 13.7)# |
| Il-5 [pg·ml−1] |
15.9 (7.6; 27.9) | 12.8 (6.7; 28.0) | 23.5 (19.2; 31.1) | 29.5 (18.1; 48.0) |
| Il-10 [pg·ml−1] |
27 (20; 48) | 22 (16; 52) | 336 (301; 487)* | 1,017 (428; 1,416)# |
| Eotaxin [pg·ml−1] |
602 (517; 736) | 857 (775; 1,292) | 1,756 (1,263; 1,992)* | 1,365 (1,000; 1,779) |
| KC [pg·ml−1] |
30 (24; 38) | 26 (19; 32) | 2,318 (1,454; 2,698)* | 1,358 (266; 2,268)# |
| Mcp-1 [pg·ml−1] |
289 (239; 349) | 239 (194; 398) | 51,720 (30,400; 96,096)* | 78,358 (4,385; 164,779)# |
| Rantes [mg·dL−1] |
72 (70; 80) | 51 (33; 59) | 4,252 (2,936; 6,277)* | 3,457 (583; 4,424)# |
| Ifn gamma [pg·ml−1] |
6 (3; 10) | 13 (6; 26) | 90 (45; 195)* | 107 (16; 152) |
*
P <0.05 vs. GR+/+ PBS.
#
P <0.05 vs. GRdim PBS. PBS = phosphate buffered saline (vehicle), 10 μl·g−1. LPS = Lipopolysaccharide from Escherichia coli (055:B5), 10 mg·kg−1. Data is shown as median (25th and 75th percentile). Bold values indicate significant differences.