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. 2019 Nov 8;15(5):865–874. doi: 10.4103/1673-5374.268902

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Copyright: © Neural Regeneration Research

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Expression of RIP3 and MLKL in cortical neurons following METH + 39°C treatment.

(A–B) Immunofluorescence staining for RIP3 and MLKL in cortical neurons: the large frame is the 4 × magnification of related small frame, scale bar: 100 μm. (C) Western blot assay for RIP3 and MLKL in cortical neurons. (D–E) Statistics of RIP3 and MLKL expression based on western blot assay (mean ± SD, n = 3, one-way analysis of variance followed by Tukey’s multiple comparison test). *P < 0.05, **P < 0.01, vs. normal group. METH: Methamphetamine; MLKL: mixed lineage kinase domain-like protein; RIP3: receptor-interacting protein 3.