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. 2019 Nov 8;15(5):948–958. doi: 10.4103/1673-5374.268930

Figure 8.

Figure 8

Macrophages in the nerve bridge expressed Slit3, while vimentin-positive cells in the nerve bridge and Schwann cells in the distal sciatic nerve expressed Robo1.

(A–D) Double staining of Slit3 (green, Alexa Fluor 488) with F4/80 (red, Alexa Fluor 568) in a transverse nerve bridge section showing that macrophages in the outermost layer of the nerve bridge express high levels of Slit3. (E–H) Double staining of Robo1 (red, Alexa Fluor 568) with the mesenchymal cell marker vimentin (green, Alexa Fluor 488) showing that vimentin-positive cells are the major cell type inside the nerve bridge expressing high levels of Robo1. (I–L) Immunopositivity of Robo1 (red, Alexa Fluor 568) in the distal nerve of PLP-GFP mice showing that Schwann cells in the distal nerve express high levels of Robo1. Ho: Hoechst. Scale bars: 120 μm in A–D, 50 μm in E–H, 20 μm in I–L. GFP: Green fluorescent protein; Ho: Hoechst 33342; PLP: myelin proteolipid protein; Robo: Roundabout.