Figure 3.

RA promoted the proliferation of oligodendrocyte progenitor cells, detected by immunofluorescence staining, in the subventricular zone of H/I injured rats 50 days after birth.

(A–D) BrdU⁺/Olig2⁺ cells in the subventricular zone. DAPI (blue) was used to stain nuclei. Representative images in the control group (A), H/I group (B), H/I + RA group (C) and H/I + vehicle group (D). (E) Quantification of Olig2⁺/BrdU⁺ cells. BrdU expression was slightly higher in the H/I group than in the control group but there was no significant change in the number of double positive cells (BrdU⁺/Olig2⁺). RA treatment after H/I injury significantly increased the number of BrdU⁺/Olig2⁺ in the subventricular zone. Data are presented as the mean ± SD (n = 6 per group). *P < 0.05, vs. control group; #P < 0.05, vs. H/I group (one-way analysis of variance followed by post hoc Tukey’s tests). H/I: Hypoxia/ischemia; RA: rosmarinic acid.