Fig. 5.

LPS treatment of alveolar bone osteocyte-like cells induces cytoskeletal alterations and the acquisition of senescence-associated SA-β-gal activity in vitro. (a) Alveolar bone osteocyte-enriched cells were prepared from 6 month-old WT female mice and treated with LPS and stained for F-Actin (green staining). (b-c) Alveolar bone osteocyte-enriched cells were cultured in supplemented α-MEM alone containing low serum (2% FBS) to reduce cell proliferation, or containing LPS (10 ng/ml). Cells were stimulated with LPS once, three times, and six times (for one day each treatment time; the shading under each image denotes the bar in panel c). The red bars denote the magnification scale (100 μm). Data represent Mean ± SEM (n=6). **P ≤ 0.001 relative to vehicle control.