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. 2020 Jan 1;17(2):145–152. doi: 10.7150/ijms.38371

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The combination of ETBF+DSS can induce colonic tumorigenesis in BALB/c mice. BALB/c mice were infected with WT-ETBF (bft-2) and subjected to the standard ETBF/AOM/DSS (2%) protocol for 12 weeks. Different combinations of reagents and/or bacteria were omitted. S, sham control with no ETBF, AOM, and DSS; E2, infected with WT-ETBF (bft-2) alone; A, AOM alone; D, DSS alone; A/D, AOM+DSS; A+E2, AOM+WT-ETBF (bft-2); D+E2, DSS+WT-ETBF (bft-2); A/D+E2, AOM+DSS+WT-ETBF (bft-2). (A) Polyp number. (B) Polyp incidence. (C) Spleen weight. (D) Colon length. Each dot represents one mouse (n = 9-15 mice per group). Horizontal bar, median. *P < 0.05, **P < 0.01, ***P < 0.001. ns, no statistical significance. nd, not detected.