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. 2020 Jan 14;17(2):224–233. doi: 10.7150/ijms.39252

Figure 1.

Figure 1

Mifepristone reduces the secretion of IL-6 and TNF-α from endometrial epithelial and stromal cells in adenomyosis. (A) Human primary endometrial epithelial cells were treated with mifepristone in different concentrations for 24h, and CCK-8 assay was performed. The viability of endometrial epithelial cells was significantly decreased when treated with mifepristone at 75 μM while there was no significant difference at 50 μM. Concentration at 50 μM was therefore selected for the following RNA-sequencing. (B) Primary endometrial epithelial cells were treated with mifepristone at the concentration of 50 μM and then subjected to next generation sequencing. The endometrial epithelial cells were from four biologically independent samples and the data were shown in quadruplicate. (C) qRT-PCR and ELISA were performed to detect the role of mifepristone on the down-regulations of IL-6 and TNF-α in endometrial epithelial and stromal cells in different concentrations. Data were shown as mean ± SEM. *P<0.05, **P<0.01 and ***P<0.001.