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. 2020 Jan 1;16(3):515–528. doi: 10.7150/ijbs.34517

Figure 2.

Figure 2

Blockade of miR-335-3p prevents the development of CNH-induced PAH and pulmonary vascular remodeling in mice. (A) Schematic diagram of the experimental protocol. MiR-335-3p antagomir treatment reversed CNH-induced increase of RVSP (B) and RV/LV+S (C). (D) qRT-PCR analysis of miR-335-3p expression in the lungs of mice after treatment of miR-335-3p antagomir. (E) Western blotting analysis of relative proteins expression in the lungs of mice with Normoxia, CNH, or CNH+antagomir treatment. (F) Quantification of proteins expression bands in (E) was done by densitometry and normalized to GAPDH. (G) Representative images of H&E staining of lung sections. (H) Representative masson trichrome staining of lung section. Values shown are means±s.e.m. N=5-8 per group. *p<0.05, **p<0.01 vs. Normoxia. #p<0.05 , ##p<0.01 vs. CNH+SCR. SCR, scramble control. PA, pulmonary artery. BR, bronchiole.