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. 2020 Jan 1;16(3):515–528. doi: 10.7150/ijbs.34517

Figure 5.

Figure 5

NF-κB is a transcriptional regulator upstream of miR-335-3p. (A) Schematic representation of putative NF-κB binding site in the promoter region of miR-335-3p, and the corresponding mutation was generated within the binding site. (B) Luciferase assay showed that the activity of the luciferase reporter fusing wild-type promoter, but not the mutated promoter with mutation within NF-κB binding site, was increased significantly after p65 cDNA treatment. **p<0.01. NC refers to an empty vector. (C) qRT-PCR analysis of pri-miR-335-3p and miR-335-3p (D) expression in the lungs of mice. Values shown are means±s.e.m. N=5-8 per group. *p<0.05 vs. Normoxia. #p<0.05 vs. CNH.