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. 2020 Jan 1;16(3):515–528. doi: 10.7150/ijbs.34517

Figure 6.

Figure 6

APJ is a direct downstream target of miR-335-3p. (A) Diagram of the miR-335-3p binding sites in the 3′-UTR of the APJ gene. (B) Luciferase reporter gene assay 3′-UTR of APJ with target and its mutant along with miR-335-3p agomir or scramble control vectors in HEK293 cells. **p<0.01. (C) Western blotting analysis of APJ expression in the lungs of mice. (D) Quantification of APJ expression bands in (C) was done by densitometry and normalized to GAPDH. Values shown are means±s.e.m. N=5-8 per group. *p<0.05 vs. Normoxia. #p<0.05 vs. CNH.