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. 2020 Jan 1;16(3):504–514. doi: 10.7150/ijbs.40435

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ITPKA interacts with Drbrin 1. A, the co-immunoprecipitation assay shown that DBN1 was able to be detected in anti-ITPKA antibody pull-down by from a total protein of H1299 cell; and in reverse co-immunoprecipitation assay, the purified pull-down protein by anti-DBN1 protein were detected by ITPKA antibody. The detection of GAPDH was used as a negative control. B, in wound healing assay, DBN1 and ITPKA knockdown could both undermine the migration ability in H1299 cells, and the double knockdown displayed the least migration ability in H1299 cells.