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. 2020 Jan 1;16(4):553–567. doi: 10.7150/ijbs.40643

Figure 2.

Figure 2

Akt2 silencing increases high glucose (HG)-induced human renal tubular epithelial (HK-2) cell apoptosis and reduces the renal protective effect of FF. Human renal tubular epithelial (HK-2) cells were treated with either control or Akt2-specific siRNA, and then the cells were treated with combinations of HG and FF for 24 h. Western blotting, an Annexin V FITC/PI Apoptosis Detection Kit, and an Accuri C6 flow cytometer and Cell Quest Pro Software were used to assess the outcomes. (A) Total Akt2 expression. (B) Apoptosis. Q1 represents dead cells, Q2 represents non-viable apoptotic cells, Q3 represents normal cells, and Q4 represents viable apoptotic cells. Quantitative analysis is shown in (C). Data were collected from at least three independent experiments and are presented as the mean ± SD (n≥5). *, p < 0.05 vs. the corresponding Con-siRNA/Ctrl; #, p < 0.05 vs. the corresponding Con-siRNA/HG; &, p < 0.05 vs. the corresponding Con-siRNA/HG/FF; +, p < 0.05 vs. the corresponding Akt2-siRNA/Ctrl; §, p < 0.05 vs. the corresponding Akt2-siRNA/HG.