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. 2020 Jan 1;16(4):553–567. doi: 10.7150/ijbs.40643

Figure 6.

Figure 6

Akt2 gene deletion partially abolishes the protective effect of FF against diabetes-associated renal damage. Renal oxidative damage was evaluated by western blotting for 4-hydroxy-2-nonenal (4-HNE) (AB). Renal inflammation was identified by western blotting for tumor necrosis factor-α (TNF-α) (AC). The severity of the renal fibrotic response was reflected in greater expression of connective tissue growth factor (CTGF), demonstrated using western blotting (AD). Masson's staining (E) was used to detect collagen fibers (blue) in kidney sections (400 ×, scale bar 100 μm). Semi-quantitative data generated from the Masson's-stained sections are presented in F. Data are presented as the mean ± SD (n≥5). *, p < 0.05 vs. the corresponding WT/Ctrl; #, p < 0.05 vs. the corresponding WT/DM; &, p < 0.05 vs. the corresponding WT/DM/FF; +, p < 0.05 vs. the corresponding Akt2-KO/Ctrl; §, p < 0.05 vs. the corresponding Akt2-KO/DM.