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. 2020 Jan 1;16(4):598–610. doi: 10.7150/ijbs.36407

Figure 1.

Figure 1

The NOTCH1-HEY1 pathway is a positive feedback loop in SACC cells. (A) The expression of NOTCH1, HEY1, TNNT3, IFI6, RELB, KRT-17, MMP7, TPD52 and ABHD3 genes, which were selected from the RNA-Seq results, was measured by qRT-PCR. (B) Linear regression analysis was used to compare the expression levels of the abovementioned genes between the RNA-Seq and qRT-PCR results. (C, D) Western blot analysis and the quantification of NOTCH1 and HEY1 expression in SACC cells after NOTCH1 upregulation by pcDNA3.1-NICD1 plasmid transfection. (E) The mRNA expression of NOTCH1 and HEY1 after HEY1 downregulation by siRNAs was detected by RT-PCR. (F, G) The protein expression of NOTCH1 and HEY1 when HEY1 inhibition was measured by western blot analysis. *P < 0.05, ** P < 0.01, and *** P < 0.005.