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. 2020 Jan 1;16(4):598–610. doi: 10.7150/ijbs.36407

Figure 3.

Figure 3

HEY1 promotes cell proliferation, self-renewal and regulates cellular apoptosis, and IMR-1 inhibits cell growth. After siRNA transfection, the cell proliferation was measured by CCK8 (A, P<0.001 by One-Way ANOVA followed by Tukey's multiple Comparison test from days 3, 4 and 5) and colony formation assays (B, P<0.01 by One-Way ANOVA followed by Tukey's multiple Comparison test, n=3). The percentages of early and late apoptosis cells were detected by flow cytometry (C, P<0.05 by One-Way ANOVA followed by Tukey's multiple Comparison test). After treated with IMR-1, the cell proliferation was measured by CCK8 (D, P<0.001 by One-Way ANOVA followed by Tukey's multiple Comparison test from days 2, 3, 4 and 5) and colony formation assays (E, P<0.01 by One-Way ANOVA followed by Tukey's multiple Comparison test, n=3). (F) Representative images of tumor spheroids of the NC, siRNA602 and siRNA1071 groups. The numbers of cell spheroids in the NC, siRNA602 and siRNA1071 groups (G, P<0.05 by One-Way ANOVA followed by Tukey's multiple Comparison test). (H) The mRNA expression of OCT4, ALDH1, Snail1, Twist1 and Fibronectin 1 when HEY1 was downregulated in SACC cells. *P < 0.05, ** P < 0.01, and *** P < 0.005.