Skip to main content
. 2020 Jan 1;16(4):611–619. doi: 10.7150/ijbs.39491

Figure 2.

Figure 2

FZU-00,003 suppresses TNBC cell proliferation and promotes apoptosis. A. FZU-00,003 suppressed HCC1937 and SUM149PT cell proliferation more efficiently than MIF in a dosage-dependent manner. DNA synthesis of FZU-00,003 or MIF treated cells were examined using the Click-iTTM EdU Alexa Fluor® 488 Imaging Kit. The quantitative results are shown on the right. B. FZU-00,003 inhibited HCC1937 and SUM149PT G1/S cell cycle progression more potently than MIF did. HCC1937 and SUM149PT cells were treated with FZU-00,003 or MIF for 24 hours. The cells were then collected and fixed for cell cycle analysis. C. FZU-00,003 induced more apoptosis in HCC1937 and SUM149PT than MIF did. HCC1937 and SUM149PT cells were treated with FZU-00,003 or MIF at indicated concentrations for 24 hours. The cells were then collected for Annexin V staining and flow cytometry analysis. *, P<0.05, **, P<0.01, t-test.