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. 2020 Jan 1;16(4):611–619. doi: 10.7150/ijbs.39491

Figure 3.

Figure 3

FZU-00,003 suppressed KLF5 expression via inducing the expression of miR-153 in TNBC cells. A. FZU-00,003 down-regulated KLF5 protein levels and induced the cleavage of PARP in a dosage-dependent manner in TNBC cells. Both HCC1937 and SUM149PT cells were treated with FZU-00,003 or MIF at indicated dosages for 24 hours. DMSO was added as the negative control. The cells were then collected for WB analysis. β-actin was used as the loading control. B. FZU-00,003 induced miR-153 expression more effectively than MIF did. FZU-00,003 or MIF treated cells were collected with Trizol for RNA extraction. Total RNA was then subjected to miRs reverse transcription and QPCR for detection of miR-153 expression. The expression of U6 was detected as the internal control.