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. 2020 Jan 1;16(4):611–619. doi: 10.7150/ijbs.39491

Figure 4.

Figure 4

Ectopic over-expression of KLF5 partially rescues FZU-00,003 induced apoptosis and cell viability reduction in HCC1937. A. KLF5 over-expression decreases FZU-00,003-induced PARP cleavage in HCC1937. HCC1937 cells were infected with pCDH-Flag -KLF5 or vector control and treated with 5μM FZU-00,003 for 24 hours. The apoptosis marker cl-PARP was detected by WB. B. Ectopic expression of KLF5 in HCC1937 partially rescued the FZU-00,003 induced cell viability reduction.HCC1937 cell were infected with pCDH-Flag-KLF5 or vector control and treated with FZU-00,003 at indicated concentrations for 48 hours before the cells were fixed for SRB assays. C. miR-153 inhibitor decreases FZU-00,003-induced KLF5 suppression and PARP cleavage in HCC1937. HCC1937 cells were transfected with miR-153 inhibitor or negative control and treated with 5μM FZU-00,003 for 24 hours. D. miR-153 inhibitor partially rescued the FZU-00,003 induced cell viability reduction in HCC1937. HCC1937 cells were transfected with miR-153 inhibitor or negative control and treated with FZU-00,003 at indicated concentrations for 48 hours before the cells were fixed for SRB assays. *, P<0.05, **, P<0.01, t-test.