Figure 3.

COR388 oral administration at a dose of 10 mg kg⁻¹ results in sustained decrease in gingipain activity and bacterial burden. (A) Dogs were dosed from day 1 to day 28; sample was taken 16 days following cessation of dosing on day 44. (B) P. gulae copy number from 2 mL saliva normalized to predose. (C) Kgp activity was assayed in GCF and SGP using activity‐based COR553 probe as described in Methods. Kgp protein was assayed by a Western blot using the polyclonal CAB102 antibody. Purified Kgp and P. gingivalis W83 lysates were run as positive controls. (D) Levels of Kgp activity normalized to individual predose values were quantified from C. (E) RgpB substrate cleavage assay in SGP samples, with values normalized to predose levels. Significance was determined using a t‐test and mean ± SEM values are shown