Figure 3. ST2 Marks a Diverse Population of KA/DP T_regs in Lung Tumor-Bearing Mice.

(A) ST2 is most highly expressed in DP lung T_regs. Representative distributions of ST2 expression on CD103⁻KLRG1⁻ (DN, gray), CD103⁺KLRG1⁻ (SP, blue), and CD103⁺KLRG1⁺ (DP, red) T_regs isolated from tumor-bearing lungs.

(B) KA_TR genes are upregulated in Il1rl1⁺ T_regs throughout tumor development. Empirical cumulative distribution functions (ECDFs) of the scores of programs 12 (top) and 21 (bottom) of Il1rl1⁺ (blue) versus Il1rl1⁻ T_regs (gray) by time point after tumor induction.

(C) Il1rl1⁺ T_regs in human colon cancer have higher expression of KA_TR genes. Overlap of genes upregulated in I11rl1⁺ T_regs in human colon cancer (blue) and programs 12 (top, p = 1.5 × 10⁻⁵) and 21 (bottom, p = 5.3 × 10⁻⁶) genes (STAR Methods). p values: hypergeometric test.

(D) IL-33 is highly expressed in lung adenocarcinoma. Immunohistochemistry (IHC) staining of tumor-bearing lungs from KP mice at weeks 13 and 22 p.i. with Lenti-LucOS. Two representative images are shown per time point. Scale bar: 20μm.

(E) Lung T_regs are enriched for ST2⁺ cells in late-stage tumors. Percent ST2⁺ among lung and msLN T_regs and T_convs from tumor-bearing KP mice at week 20 p.i. as measured by flow cytometry. Error bars: SEM. ****p < 0.0001, *p < 0.05, Tukey’s multiple comparisons test.