KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| KLRG1 2F1 PE-Cy7 | Thermo Fisher | RRID: AB_1518768 |
| CD103 2E7 APC | BioLegend | RRID: AB_1227502 |
| CD4 RM4-5 APC-eFluor780 | Thermo Fisher | RRID: AB_1272183 |
| Foxp3 FJK-16 s FITC | Thermo Fisher | RRID: AB_465243 |
| IL-17 eBio17B7 PerCP-Cy5.5 | Thermo Fisher | RRID: AB_925753 |
| CD44 IM7 Alexa Fluor 700 | Thermo Fisher | RRID: AB_494011 |
| CD62L MEL-14 eFluor450 | Thermo Fisher | RRID: AB_1963590 |
| CCR6 29-2L17 PE/Dazzle 594 | BioLegend | RRID: AB_2687019 |
| RORgt Q31-378 Alexa Fluor 647 | BD Biosciences | RRID: AB_2738916 |
| T-bet O4-46 PE | BD Biosciences | RRID: AB_10564071 |
| PD-1 J43 PE-Cy7 | BioLegend | RRID: AB_572017 |
| CD69 H1.2F3 BV785 | BioLegend | RRID: AB_2629640 |
| CXCR3 CXCR3-173 BV421 | BD Biosciences | RRID: AB_10900974 |
| ST2 U29-93 Brilliant Blue 700 | BD Biosciences | RRID: AB_2743483 |
| CD85k H1.1 PE | Biolegend | RRID: AB_2561653 |
| Ki-67 B56 BV786 | BD Biosciences | RRID: AB_2732007 |
| CD45.2 104 V500 | BD Biosciences | RRID: AB_10897142 |
| Thy1.2 30-H12 APC-eFluor780 | Thermo Fisher | RRID: AB_1272187 |
| CD103 2E7 BV510 | BioLegend | RRID: AB_2562713 |
| CD4 RM4-5 BUV737 | BD Biosciences | RRID: AB_2738734 |
| CD8a 53-6.7 BUV395 | BD Biosciences | RRID: AB_2739421 |
| CD45 30-F11 PE-CF594 | BD Biosciences | RRID: AB_11154401 |
| CD45 30-F11 APC-Ef780 | Thermo Fisher | RRID: AB_1548781 |
| CXCR6 SA051D1 PE/Dazzle 594 | Biolegend | RRID: AB_2721700 |
| KLRG1 2F1 BV711 | BioLegend | RRID: AB_2629721 |
| CD11c HL3 PE-Cy7 | BD Biosciences | RRID: AB_469590 |
| Siglec F E50-2440 PE | BD Biosciences | RRID: AB_394341 |
| CD4 RM4-4 PE | Biolegend | RRID: AB_313691 |
| CD8b eBioH35-17.2 PE | Thermo Fisher | RRID: AB_657768 |
| IHC and IF: Goat anti-mouse IL-33 | R&D Biosystems | RRID: AB_884269 |
| IHC: Rat anti-mouse CD8alpha | Thermo Fisher | RRID: AB_2637159 |
| IF: Rabbit anti-mouse proSP-C | Millipore | RRID: AB_91588 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant mouse IL-33 | BioLegend | Biolegend 580506 |
| Cell Stimulation Cocktail | Thermo Fisher | Cat: 00-4970-03 |
| SIINFEKL-Kb monomer | NIH Tetramer Core | |
| Critical Commercial Assays | ||
| Thru-Plex-FD Library Prep Kit | Rubicon Genomics | |
| Nextera XT Library Prep Kit | Illumina | FC-131-1096 |
| Deposited Data | ||
| Bulk and single cell RNA sequencing | GEO | GEO: GSE139232 |
| Experimental Models: Organisms/Strains | ||
| Mouse: B6.129S4-Krastm4Tyj/J | RRID: IMSR_JAX:008179 | |
| Mouse: B6.129P2-Trp53tm1Brn/J | Jackson Laboratory | RRID: IMSR_JAX:008462 |
| Mouse: C57BL/6-Foxp3tm1Flv/J | RRID: IMSR_JAX:008374 | |
| Mouse: Foxp3tm1Kuch | Bettelli et al., 2006 | MGI:3718527 |
| Mouse: B6.129(Cg)-Foxp3tm4(YFP/icre)Ayr/J | Jackson Laboratory | RRID: IMSR_JAX:016959 |
| Mouse: Il1rl1tm1.1Rlee | Chen et al., 2015 | MGI:5818148 |
| Oligonucleotides | ||
| See Table S7. | This paper | N/A |
| Recombinant DNA | ||
| Plasmid: Lenti-LucOS | Addgene | Addgene_22777 |
| Plasmid: pGK::GFP-LucOS::EFS::FlpO (FlpO-GFP-OS) | This paper | Available upon request |
| Software and Algorithms | ||
| Code generated for this study | This paper | Available upon request |
| SCDE | Fan et al., 2016 | http://hms-dbmi.github.io/scde/ |
| Bowtie | Langmead et al., 2009 | http://bowtie-bio.sourceforge.net/bowtie2/index.shtml |
| RSEM v1.2.8 | Li and Dewey, 2011 | https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-12-323 |
| JADE v1.1.0 | Miettinen et al. 2017 | https://cran.r-project.org/web/packages/JADE/index.html |
| Tracer | Stubbington et al., 2016 | https://github.com/Teichlab/tracer |
| Gephi | Bastian et al., 2009 | https://gephi.org |
| EnrichmentMap (Cytoscape) | Merico et al., 2010 | https://www.cytoscape.org |
| QuPath | Bankhead et al., 2017 | https://qupath.github.io/ |
| Prism | GraphPad |