Table 1.

Hybrid and Homogeneous PP7 Virus-like Particles

entry	VLPa	incorporationb	Rhc (nm)
1	PP7 + Z-PP7-Z	40 per capsid	20.8 ± 0.1
2	PP7 + ZZ-PP7	90 per capsid	21.7 ± 1.1
3	PP7 + PP7-ZZ	40 per capsid	21.0 ± 1.8
4	PP7-ZZ	180 per capsid	21.5 ± 0.3
5	ZZ-PP7	180 per capsid	20.9 ± 0.4
6	PP7-PP7-ZZ	120 per capsidd	22.6 ± 1.4

^a“Z“ = A M V D N K F N K E Q Q N A F Y E I L H L P N L N E E - QRNAFIQSLKDDPSQSANLLAEAKKLNDAQAPK. “ZZ” = Z-AYGG-Z.

^bAverage number of extended coat proteins per VLP, determined for the hybrid particles (entries 1–3) by relative peak intensities in LC-MS spectra shown in Figure S1, assuming that both wild-type and extended polypeptides are detected with the same sensitivity.

^cHydrodynamic radius measured by dynamic light scattering; see Figure S1 for representative measurement.

^dSee text.