Table 2.
Successful C-Terminal Extension Modifications of the PP7 Virus-like Particle
| entry | VLPa | description or function | peptide sequences | Rhb (nm) |
|---|---|---|---|---|
| 1 | M, D | ZZ-domain (ZZ) | [see caption to Table 1] | 21.5 ± 0.3, 22.6 ± 1.4 |
| 2 | M, D | transferrin recognition (TfR) | HAIYPRH | 17.4 ± 0.8, 18.0 ± 0.2 |
| 3 | M, D | EGFR recognition (GE7) | NPVVGYIGERPQYRDL | 17.8 ± 0.3, 18.7 ± 1.2 |
| 4 | M, D | OVA1 antigen (OVA1) | SIINFEKL | 19.0 ± 1.5, 18.8 ± 1.2 |
| 5 | M, D | OVA2 antigen (OVA2) | ISQAVHAAHAEINEAGR | 18.8 ± 1.1, 18.5 ± 0.9 |
| 6 | D | Trypanosoma trans-sialidase epitope (TS) | ATIENRDVM | 18.0 ± 0.2 |
| 7 | D | P. falciparum circumsporozoite protein (NANP) | NANPNVDPNANPNANPNANPNANP | 20.0 ± 0.2 |
a
M = fusion to the PP7 monomeric coat protein; D = fusion to the PP7-PP7 dimeric coat protein.
b
Hydrodynamic radii measured by DLS (M, then D); see Figures S3 and S4 for representative measurements.