Skip to main content
. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2019 May 29;378:114602. doi: 10.1016/j.taap.2019.114602

Figure 3. Peroxynitrite contributes to AsIII-induced dysfunction and zinc loss from PARP-1.

Figure 3.

A) PARP-1 activity restoration by scavenging peroxynitrite. HEKn cells were treated by 10 μM SIN-1 (peroxynitrite donor), 1 μM AsIII, or co-treated with 100 μM Uric Acid for 24 h. PARP-1 activity was then measured as described in the methods section. Scavenging peroxynitrite significantly rescued AsIII-inhibited PARP-1 activity. Bar charts show mean ± SD. *, p < 0.05, n = 3. B) Scavenging peroxynitrite restored AsIII-induced zinc loss on PARP-1 protein. HEKn cells were treated as described in panel A. Zinc content in PARP-1 protein was then measured as described in the methods section. Scavenging peroxynitrite restored zinc loss induced by AsIII. *, p < 0.05, n = 3.