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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2019 May 29;378:114602. doi: 10.1016/j.taap.2019.114602

Figure 4. AsIII-induced PARP-1 S-nitrosation through peroxynitrite production.

Figure 4.

A) AsIII-induced PARP-1 S-nitrosation in a peroxynitrite-dependent manner. HEKn cells were treated for 24 h with 10 μM SIN-1 (peroxynitrite donor), 1 μM AsIII, or co-treated with 100 μM Uric Acid. PARP-1 S-nitrosation was measured by modified biotin-switch assay and immunoblotting. B) Densitometric analysis of immunoblotting for PARP-1 S-nitrosation. SIN-1 and AsIII induced PARP-1 S-nitrosation. Scavenging peroxynitrite significantly reduced PARP-1 S-nitrosation. Bar chart shows mean ± SD. *, p < 0.05, n = 3.