Fig. 2. Cell cycle analysis of BEAS-2B cells treated with prometryn (0, 25, 50, 100, 200 μM) for 48 h. The treated cells were harvested and labeled with PI, then analyzed by flow cytometry (a). Quantitative data of cell cycle distribution. Results are representative of at least three independent experiments. *, P < 0.05, **, P < 0.01, ***, P < 0.001, versus vehicle-only control (Student's t-test) (b). Western blot detection of P53 and cell cycle-related proteins in BEAS-2B cells, with GAPDH used as the internal reference (c).