Figure 5.

miR‐18a inhibits autophagy in RAW264.7 macrophages by suppressing ATM. A, The expression of LC3‐II in RAW264.7 macrophages transfected with miR‐18a mimics, NC‐mimics, miR‐18a inhibitor or NC‐inhibitor followed by Mtb infection. B, The binding sites and matching degree were predicted between miR‐18a and ATM by TargetScan analysis. C, The expression of ATM mRNA at different time‐points in RAW264.7 macrophages infected with Mtb by RT‐PCR. D, Correlation analysis between miR‐18a and ATM expression. E, The expression of ATM mRNA in RAW264.7 macrophages transfected with miR‐18a mimics, NC‐mimics, miR‐18a inhibitor or NC‐inhibitor followed by Mtb infection by RT‐PCR. F, The expression of ATM protein in RAW264.7 macrophages transfected with miR‐18a mimics, NC‐mimics, miR‐18a inhibitor or NC‐inhibitor followed by Mtb infection by Western blot. G, Observation of ATM immunofluorescence by laser scanning confocal microscope. Data represent the means ± SD from three independent experiments. **P < .01, ***P < .001. Relative ATM expression, ATM/β‐actin