Figure 2.

Allopurinol increased the cardiac expression and nuclear translocation of Nrf2 and its down ARE (HO‐1, p62). A‐C, Cardiac expression of total, nuclear and cytosolic Nrf2. D, Cardiac Keap1 protein expression. E, F, Cardiac HO‐1 and p62 protein expression. G, The ratio of LC3 II/LC3 I in the myocardium. H‐J, Cardiac mRNA expression of Nrf2, Keap1 and p62, respectively, calculated against the housekeeping gene GAPDH. Data are shown as mean ± SEM, with n = 6 animals per group. *P < .05 vs C; ^# P < .05 vs DA; H3, histone H3