Figure 4.

Nrf2/p62 signalling pathway played the key role in the ALP protection to high glucose‐induced cardiomyopathy. A, B, Protein and mRNA expression of Nrf2 after siRNA treatment in the normal glucose. C, Protein expression of Nrf2. D, Protein expression of Keap1. E, Protein expression of p62. F, The ratio of LC3 II/LC3 I in the H9C2 cells. G‐I, The mRNA expressions of Nrf2, p62 and Keap1, respectively, in H9C2 cells, calculated against the housekeeping gene GAPDH. J, Nuclear Nrf2 expression in high glucose cultured H9C2 with or without ALP. K, Treatment with Bafilomycin (Baf), a lysosomal inhibitor that can impair autophagosome‐lysosome fusion and thus track autophagic flux, induced higher P62 protein level than NG group, whereas HG group displayed decreased P62 protein level than NG group. Data are mean ± SEM of two independent experiments each performed in triplicate, *P < .05. vs NG; ^# P < .05 vs HG; ^$ P < .05 vs HA; ^& P < .05 vs Nrf2