Figure 4.

Overexpression of WT1 partially prevents degrasyn‐induced anti‐cancer activity. (A) PANC‐1 and BxPC‐3 cells were transduced with retroviral negative control (MSCV‐NC) or MSCV‐WT1, which overexpresses WT1. Positive clones were selected by puromycin for seven days. Transduced‐PANC‐1 and BxPC‐3 cells were treated with or without 1.0 μM degrasyn for 24 hours, and WT1 expression was detected by Western blot. (B and C) Transwell migration assays were performed in PANC‐1 (B) and BxPC‐3 cells (C), which were transduced with MSCV‐NC or MSCV‐WT1 and treated with or without 1.0 μM degrasyn for 24 hours. ** and ^## P < .01. (D and E) Colony formation was performed by methylene blue stain in PANC‐1 (D) and BxPC‐3 cells (E), which were transduced with MSCV‐NC or MSCV‐WT1 followed by 1.0 μM degrasyn treatment or not for 24 hours. ** and ^## P < .01