Figure 6.
rhCCN1 injection promotes the skin growth and improves the efficiency of skin expansion. Rat skin was expanded as described in the Methods, and the entire expanded area was injected with rhCCN1 (0.1 µg/cm2, i.c., daily) or same amount of BSA (0.1 µg/cm2, i.c., daily). The rats were sacrificed on day 7. A, Skin tissue blood perfusion was analysed by laser speckle contrast analysis (LASCA) on day 7 (n = 5 per group). B, Ultrasound test on day 7 (n = 5 per group). C, Masson trichrome staining of the expanded skin tissues from both groups (n = 5 per group, scale bar: 1000 µm). Skin thickness was measured by Image J. D, Immunostaining of KRT5 (green), KRT10 (red) and Vimentin (green) of the expanded skin tissues from both groups (n = 5 per group, scale bar: 50 µm). All values represent the means ± SD of triplicate determinations. ***P < .001