Figure 5.

The ERK1/2 pathway involves in NFAT5‐mediated chemotaxis of THP‐1 cells. A, Representative images of migrated THP‐1 cells supplemented with the culture supernatant of HUVECs which had been pretreated with ERK1/2 inhibitor (U0126, 10 μmol/L) or DMSO for 30 min before treatment with IL‐1β (10 ng/mL) for an additional 12 h. B, The number of migrated THP‐1 cells on the reverse side of the membrane and in the lower medium. C‐E, ERK1/2, NFAT5 and MCP‐1 protein expressions in HUVECs treated as (A). *P < .05, **P < .01 vs + DMSO; ^## P < .01 vs control. Each experiment was repeated three times