Figure 1.

10 × Genomics single‐cell technology enables the profiling of RNAs from thousands of single cells simultaneously. Cells were combined with reagents in one channel of a chip. Reverse transcription took place inside each GEM, after which cDNAs were pooled to perform amplification and library construction in bulk. Gel beads loaded with primers and barcoded oligonucleotides were first mixed with cells and reagents, and subsequently mixed with oil‐surfactant solution at a microfluidic junction. Single‐cell GEMs were collected in the GEM outlet. Finished library molecules consisted of Illumina adapters and sample indices, which allowed for pooling and sequencing of multiple libraries on a next‐generation short read sequencer