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. 2019 Dec 17;24(2):1945–1957. doi: 10.1111/jcmm.14891

Table 1.

Summary of single cells sequencing by 10 × Genomics

  hUC‐MSCs hSF‐MSCs
Cells
Estimated number of cells 1597 1259
Fraction reads in cells 81.2% 77.8%
Mean reads per cell 65 598 52 245
Median genes per cell 3333 2954
Total genes detected 17 317 16 996
Median UMI counts per cell 18 304 12 609
Sequencing
Number of reads 104 761 163 65 777 15
Valid barcodes 86.2% 70.4%
Reads mapped confidently to transcriptome 78.2% 60.4%
Reads mapped confidently to exonic regions 81.8% 63.1%
Reads mapped confidently to intronic regions 5.9% 8.1%
Reads mapped confidently to intergenic regions 2.1% 2.6%
Sequencing saturation 43.4% 18.1%
Q30 bases in barcode 47.2% 29.6%
Q30 bases in RNA read 89.2% 62.2%
Q30 bases in sample index 89.0% 70.9%
Q30 bases in UMI 92.6% 67.5%
Sample
Transcriptome   GRCh38
Chemistry   Single Cell 3' v1
Cell ranger version   1.2.0