Table 1.
Summary of single cells sequencing by 10 × Genomics
hUC‐MSCs | hSF‐MSCs | |
---|---|---|
Cells | ||
Estimated number of cells | 1597 | 1259 |
Fraction reads in cells | 81.2% | 77.8% |
Mean reads per cell | 65 598 | 52 245 |
Median genes per cell | 3333 | 2954 |
Total genes detected | 17 317 | 16 996 |
Median UMI counts per cell | 18 304 | 12 609 |
Sequencing | ||
Number of reads | 104 761 163 | 65 777 15 |
Valid barcodes | 86.2% | 70.4% |
Reads mapped confidently to transcriptome | 78.2% | 60.4% |
Reads mapped confidently to exonic regions | 81.8% | 63.1% |
Reads mapped confidently to intronic regions | 5.9% | 8.1% |
Reads mapped confidently to intergenic regions | 2.1% | 2.6% |
Sequencing saturation | 43.4% | 18.1% |
Q30 bases in barcode | 47.2% | 29.6% |
Q30 bases in RNA read | 89.2% | 62.2% |
Q30 bases in sample index | 89.0% | 70.9% |
Q30 bases in UMI | 92.6% | 67.5% |
Sample | ||
Transcriptome | GRCh38 | |
Chemistry | Single Cell 3' v1 | |
Cell ranger version | 1.2.0 |