Figure 3.

Effect of KLF4 on the mesenchymal properties of Lgr5⁺CD44⁺EpCAM⁺ cells. A‐B, KLF4 knockdown resulted in decreased expression of N‐cad, Vim, Snail, Slug, but increased expression of E‐cad and ZO‐1 mRNA and protein. C, Tumour cell migration and invasion capabilities were suppressed after KLF4 knockdown as detected by real‐time migration and invasion assay. D, KLF4 overexpression increased N‐cad, Vim, Snail, Slug expression, and decreased expression of E‐cad and ZO‐1. E, Tumour cell migration and invasion capabilities increased after KLF4 overexpression as detected by using the real‐time migration and invasion assay. The data represented as mean ± SD of three replicated experiments (*P < .05)