Figure 2.

Amurensin H (AH) inhibited IL‐1β‐induced inflammatory mediators in rat chondrocytes. Chondrocytes were treated with IL‐1β for 24 h (A, B) or 48 h (C‐F) after pre‐incubation with AH for 2 h (n = 6). (A‐C) Cytosolic iNOS expression and NO level were attenuated by AH treatment. (A, B and D) Cytosolic COX‐2 expression and PGE2 level were attenuated by AH treatment. (E, F) Levels of IL‐6, TNF‐α and IL‐17 were decreased after AH treatment. β‐Actin was used as a control for equal loading. #P < .05 and ##P < .01 vs control group; *P < .05 and **P < .01 vs IL‐1β‐treated group