Figure 6.

Amurensin H (AH) inhibited downstream pro‐inflammatory transcription factor NF‐κB in rat chondrocytes. Chondrocytes were treated with IL‐1β for indicated time‐points (A) or 1 h (B, C) after pre‐incubation with AH for 2 h (n = 3). (A) AH inhibited p65 phosphorylation in a time‐dependent manner. (B, C, D) AH inhibited p65 phosphorylation and p65 (F, 200×) translocation in a dose‐dependent manner. β‐Actin was used as a control for equal loading. #P < .05 and ##P < .01 vs control group; *P < .05 and **P < .01 vs IL‐1β‐treated group