Figure 3.

Anticancer drugs induced mitochondrial membrane insertion of BNIP3, resulting in the generation of ROS and cell death. A, A549 cells (2 × 10⁵) were transfected with pcDNA empty vector or ΔTM‐BNIP3 plasmid. Sixteen hours later, cells were treated with cisplatin (2 μg/mL), LBH589 (100 nmol/L), or rapamycin (100 nmol/L), or a combination of two drugs for 48 h. Cell viability was analysed using a Celltiter‐Glo luminescent cell viability assay. B, A549 cells were treated with cisplatin (2 μg/mL), LBH589 (100 nmol/L), or rapamycin (100 nmol/L), or a combination of two drugs for 48 h. Cells were treated with or without the ROS inhibitor NAC (5 mmol/L) 24 h prior to analysis. Cell viability was analysed using a Celltiter‐Glo luminescent cell viability assay. Results are representative of at least three independent experiments. *P < .05 and **P < .01