Figure 2.

Combined treatment with JQ1 and venetoclax exerts synergistic lethal activities against CLL cell lines. A, B, MEC‐1 (left) and EHEB (right) cells were cultured in the presence or absence of JQ1 and venetoclax at indicated concentration. Cell counts were measured every 48 h for 144 h by CTG assay. C, D, Bcl2 protein expression MEC‐1 (C) and EHEB (D) upon treatment with venetoclax, JQ1 and the association. E, F, MEC‐1 (left) and EHEB (right) cells were treated with JQ1 and venetoclax for 48 h. The percent of Annexin V/PI‐positive apoptotic cells was determined by flow cytometry. Columns, mean of 3 independent experiments. G, H, Representative immunoblots of MEC‐1 and EHEB cells treated with the JQ1 for 48 h. Immunoblot analyses were conducted for the expression levels of p‐ERK1/2, ERK1/2, p‐AKT, AKT and vinculin in the cell lysates