Figure 3.

Overexpression of LASP1 in HNSCC cells promotes cell proliferation, migration and invasion and enhances the G2/M stage of the cell cycle. A, Western blot and real‐time PCR were used to detect the LASP1 overexpression efficiency after the lentivirus‐OERNA infection of HNSCC cells. B, Cell proliferation at 6 d was measured by CCK‐8 following the overexpression of LASP1. C, A plate cloning formation assay was performed to investigate colony forming after the overexpression of LASP1; the number of clones containing >50 cells was counted. D, A transwell analysis was performed to measure metastasis and invasion following the overexpression of LASP1 in the HNSCC cells. E, The representative FACS results indicate the cell cycle changes following the overexpression of LASP1. The quantitative results were analysed in three independent experiments, ***P < .05, ****P < .01 compared with the control group