Figure 5.

LASP1 and HSPA1A bind directly with each other and are co‐in the cytoplasm. A, A Co‐IP assay followed by LC/MS/MS examination was performed to detect the directly interactive binding partner of LASP1 in the CAL27 cells. The most obvious single band was detected at 70 kd. B, As indicated by mass spectrometry, the protein interactive with LASP1 was HSPA1A. C, The results of the co‐IP assay indicate that LASP1 directly binds with HSPA1A. D, The results of the co‐IP assay showed that HSPA1A is also closely interactive with LASP1. E, The subcellular localization of HSPA1A and LASP1 in the CAL27 cells was assessed by immunofluorescence staining