Figure 3.
Regulatory effect of nucleolin on the expression and secretion of EGF and PDGF‐BB (A) The effect of nucleolin overexpression on the expressions of nucleolin, EGF and PDGF‐BB; (B) the effect of nucleolin overexpression on Ang II‐induced expression and secretion of EGF and PDGF‐BB. C, The effect of nucleolin low expression on expressions of nucleolin, EGF and PDGF‐BB. D, The effect of nucleolin low expression on Ang II‐induced expression and secretion of EGF and PDGF‐BB. VSMCs were transfected with the control plasmid (pcDNA3.1 or psiRNA) and the recombinant plasmid (pcDNA3.1‐Nuc or psiRNA‐Nuc), cells were treated with 10−5 mmol/L Ang Ⅱ for 48 h, the total protein was extracted from the transfected cells, and then RT‐qPCR, Western blotting and ELISA were performed. Ang II: AngII treatment; pcDNA3.1 and psiRNA: control plasmid; pcDNA3.1‐Nuc and psiRNA‐Nuc: nucleolin overexpression plasmid and nucleolin RNA interference plasmid, β‐actin was used as an internal control. (Data were expressed as ± S, n = 3; * P < .05 vs the control group; # P < .05 vs the untransfected group and control plasmid group)