Figure 4.

Protective effect of baicalein on H₂O₂ induced cardiotoxicity. H9C2 cells were pre‐treated with 50 μM baicalein for 4 h, then H9C2 cells were exposed to 100 μM H₂O₂ for 24 h, and the expression level of MARCH5 was detected by Western blotting (A). The percentage of cells undergoing mitochondrial fission is shown as (B). The percentage of apoptotic cells is shown as (C) detected by TUNEL assay. The numbers of autolysosomes and autophagosomes were observed as (D). LC3 protein was detected by Western blotting (E) and densitometry (F). All of the data were expressed as the mean ± SEM of three independent experiments. *P < .05, **P < .01, ***P < .001