Central administration of RFRP-3 suppresses pituitary Lhβ subunit mRNA expression in the afternoon but not the morning (top; n = 6, 6, 6, 7, for AM saline, AM RFRP-3, PM saline, PM RFRP-3, respectively). Pituitary Gnrh-r mRNA expression is not affected by either RFRP-3 or time of day (middle; n = 6, 8, 7, 7, for AM saline, AM RFRP-3, PM saline, PM RFRP-r, respectively). RFRP-3 suppresses pituitary Gpr147 mRNA expression in the afternoon but not the morning (bottom; n = 6, 8, 9, 5, for AM saline, AM RFRP-3, PM saline, PM RFRP-3, respectively). Data are presented as mean ± standard error of the mean. * p < 0.05.