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mPOA Gnrh mRNA expression is suppressed by central administration of RFRP-3 in the morning but not in the afternoon (top; n = 6, 6, 7, 9, for AM saline, AM RFRP-3, PM saline, PM RFRP-3, respectively). RFRP-3 does not alter mPOA Gpr147 mRNA expression (bottom; n = 7, 5, 7, 8, for AM saline, AM RFRP-3, PM saline, PM RFRP-r, respectively). Data are presented as mean ± standard error of the mean. * p < 0.05.
